CHARACTERIZATION OF GLUCOSE TRANSPORT BY CULTURED RABBIT KIDNEY PROXIMAL CONVOLUTED AND PROXIMAL STRAIGHT TUBULE CELLS

2002 ◽  
Vol 38 (4) ◽  
pp. 218 ◽  
Author(s):  
PEDRO L. DEL VALLE ◽  
ANNA TRIFILLIS ◽  
CHARLES E. RUEGG ◽  
ANDREW S. KANE
Keyword(s):  
Glucose Transport ◽  
Rabbit Kidney ◽  
Straight Tubule ◽  
Proximal Straight Tubule ◽  
Tubule Cells

Vitamin D3 metabolites increase [Ca2+]i in rabbit renal proximal straight tubule cells

1991 ◽  
Vol 260 (5) ◽  
pp. F757-F763 ◽  
Author(s):  
M. Suzuki ◽  
S. Kurihara ◽  
Y. Kawaguchi ◽  
O. Sakai
Keyword(s):  
Vitamin D ◽  
Proximal Tubule ◽  
Ion Concentration ◽  
Rabbit Kidney ◽  
Vitamin D Metabolites ◽  
Dihydroxyvitamin D3 ◽  
Straight Tubule ◽  
Proximal Straight Tubule ◽  
Tubule Cells ◽  
Free Media

Vitamin D metabolites exert both acute and chronic influences on proximal tubule function. To further evaluate vitamin D action on the kidney, we examined the immediate effects of vitamin D metabolites on cytoplasmic calcium ion concentration [( Ca2+]i), using fura-2 and patch-clamp method in cultured proximal straight tubule cells of rabbit kidney. 1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] and 25-hydroxyvitamin D3 [25(OH)D3] evoked a transient rise in [Ca2+]i, and 24,25-dihydroxyvitamin D3 [24,25(OH)2D3] caused a sustained rise in [Ca2+]i; all effects were dose dependent. [Ca2+]i transient, evoked by 1,25(OH)2D3 alone, was abolished in Ca(2+)-free media. Pretreatment of cells in Ca(2+)-free media with caffeine (4 mM) or ryanodine (1 microM) to deplete Ca2+ store of endoplasmic reticulum or with TMB-8 (5 mM) to block Ca2+ release from storage blunted the effect of 25(OH)D3 on [Ca2+]i but not of 24,25(OH)2D3. Data were also supported by activities of Ca-dependent K channel and show that these three vitamin D metabolites in pharmacological doses increase [Ca2+]i of proximal tubule cells from different sources.

Hydrogen Peroxide Induces Necrosis, Apoptosis, Oncosis and Apoptotic Oncosis of Mouse Terminal Proximal Straight Tubule Cells

Nephron ◽  
1999 ◽  
Vol 81 (2) ◽  
pp. 234-238 ◽  
Author(s):  
Michio Takeda ◽  
Isao Shirato ◽  
Mami Kobayashi ◽  
Hitoshi Endou
Keyword(s):  
Hydrogen Peroxide ◽  
Straight Tubule ◽  
Proximal Straight Tubule ◽  
Tubule Cells

Cyclosporine Induces Apoptosis of Mouse Terminal Proximal Straight Tubule Cells

Nephron ◽  
1998 ◽  
Vol 80 (1) ◽  
pp. 121-122 ◽  
Author(s):  
Michio Takeda ◽  
Mami Kobayashi ◽  
Isao Shirato ◽  
Hitoshi Endou
Keyword(s):  
Straight Tubule ◽  
Proximal Straight Tubule ◽  
Tubule Cells

Immunocytochemical characterization of Na(+)-H+ exchanger isoform NHE-1 in rabbit kidney

1992 ◽  
Vol 263 (5) ◽  
pp. F833-F840 ◽  
Author(s):  
D. Biemesderfer ◽  
R. F. Reilly ◽  
M. Exner ◽  
P. Igarashi ◽  
P. S. Aronson
Keyword(s):  
Fusion Protein ◽  
Renal Cortex ◽  
Apical Membrane ◽  
Basolateral Membrane ◽  
Rabbit Kidney ◽  
Thick Ascending Limb ◽  
Nephron Segments ◽  
Basolateral Plasma Membrane ◽  
Tubule Cells

We have recently isolated cDNAs encoding a Na(+)-H+ exchanger isoform, referred to as NHE-1, from rabbit kidney and LLC-PK1 cells. To identify the NHE-1 protein and to establish its cellular and subcellular localization in the rabbit kidney, we prepared antibodies to a NHE-1 fusion protein. cDNA encoding the COOH-terminal 41 amino acids of NHE-1 was subcloned into a maltose-binding protein vector and the purified fusion protein (FP347A) used to immunize guinea pigs. To identify the NHE-1 protein, we performed Western blot analysis against membrane fractions prepared from rabbit renal cortex. Anti-FP347A antibody specifically reacted with a polypeptide with an apparent molecular mass of 100–110 kDa that was enriched in basolateral membrane fractions. When indirect immunofluorescence was performed on semithin (0.5 micron) cryosections of paraformaldehyde-lysine-periodate-fixed rabbit kidney, anti-FP347A specifically stained the basolateral plasma membrane of cells of the proximal tubule, thick ascending limb, and distal convoluted tubule. Anti-FP347A similarly stained connecting tubule cells and principal cells. No staining was detected on the apical membrane of any cells of the rabbit nephron. We conclude that NHE-1 is a 100- to 110-kDa protein expressed on the basolateral membrane of multiple nephron segments.

Glucose transport in chronically altered rat nephrons

1982 ◽  
Vol 243 (4) ◽  
pp. F393-F403
Author(s):  
R. A. Kramp ◽  
W. B. Lorentz
Keyword(s):  
Glucose Transport ◽  
Absorptive Capacity ◽  
Compensatory Growth ◽  
Glucose Load ◽  
Straight Tubule ◽  
Anesthetized Rats ◽  
Normal Configuration ◽  
Proximal Straight Tubule ◽  
Different Levels

Microinjection experiments were performed in anesthetized rats to study the tubular absorptive capacity for glucose (TG) in normal and chronically altered tubules, i.e., tubules that sustained anatomical repair and compensatory changes of their previously normal configuration after acute damage. TG was complete when the glucose load injected in normal or altered early or late proximal convolutions was less than or equal to 1.5 or less than or equal to 10 pmol . s-1, respectively. In normal tubules, TmG was 10 and 5 pmol . s-1 after early and late proximal microinjections, respectively. After early proximal microinjections in altered tubules, different levels of TmG were found. They related to the degree of compensatory growth. TmG was approximately 12, 24, or 35 pmol . s-1, respectively, in altered tubules with minor, moderate, or major compensatory growth. After late proximal microinjections, TmG was 15 pmol . s-1. TG variability was greater in altered than in normal tubules. Correlation between structural and functional compensatory changes was thus demonstrated. Furthermore, an important, albeit latent, absorptive capacity for glucose of the proximal straight tubule was found.

scholarly journals Selective depletion of mouse kidney proximal straight tubule cells causes acute kidney injury

2011 ◽  
Vol 21 (1) ◽  
pp. 51-62 ◽  
Author(s):  
Michiko Sekine ◽  
Toshiaki Monkawa ◽  
Ryuji Morizane ◽  
Kunie Matsuoka ◽  
Choji Taya ◽  
...  
Keyword(s):  
Acute Kidney Injury ◽  
Kidney Injury ◽  
Mouse Kidney ◽  
Straight Tubule ◽  
Selective Depletion ◽  
Proximal Straight Tubule ◽  
Tubule Cells

Potassium secretion in the rabbit proximal straight tubule

1983 ◽  
Vol 245 (2) ◽  
pp. F167-F174 ◽  
Author(s):  
A. G. Wasserstein ◽  
Z. S. Agus
Keyword(s):  
Rabbit Kidney ◽  
Renal Handling ◽  
Straight Tubule ◽  
Proximal Straight Tubule ◽  
Proximal Tubular ◽  
K Secretion ◽  
Luminal Flow ◽  
Potassium Secretion ◽  
K Concentration

The renal handling of potassium is generally thought to involve proximal reabsorption and distal secretion. To evaluate transport in the pars recta, we perfused S2 and S3 segments from superficial and juxtamedullary proximal straight tubules isolated from the rabbit kidney. The data indicate net potassium secretion in the isolated perfused perfused proximal straight tubule (PST). K+ secretion (JK, pmol X mm-1 X min-1) was -2.51 +/- 0.53 in superficial PST S2 segments, -2.80 +/- 1.05 in superficial PST S3 segments, and -1.36 +/- 0.84 in juxtamedullary PST. Secretion was inhibited by 10(-5) M ouabain in the bath in superficial S2 and S3 segments. When a solution resembling late proximal tubular fluid was perfused in superficial PST, JK fell from -3.86 +/- 1.77 to -0.45 +/- 0.63 pmol X mm-1 X min-1. When luminal flow rate was varied in the physiologic range in individual superficial S2 and S3 segments, JK varied directly; K+ secretion increased by -0.5 pmol X mm-1 X min-1 per 1 nl X min-1 increment in luminal flow, while collected K+ concentration did not vary significantly. When a favorable bath-to-lumen K+ gradient (10 vs. 5 mM) was imposed, K+ secretion was markedly enhanced; when an equal but oppositely directed gradient was imposed, net K+ reabsorption was observed. These data are consistent with a gradient-limited process. In midcortical tubule segments (S2 and S3), 10(-3) M amiloride in perfusate inhibited net K+ secretion from -2.77 +/- 0.52 to -0.18 +/- 1.08 pmol X mm-1 X min-1 and fluid absorption from 0.42 +/- 0.10 to 0.18 +/- 0.05 nl X mm-1 X min-1. Net K+ secretion in S2 and S3 segments of PST may contribute to previously reported K+ secretion prior to the bend of Henle's loop. The magnitude of this process in vivo is uncertain in the absence of measurements of interstitial K+ concentration in the milieu of the PST.

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